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. 2019 Apr 17;14(4):e0215713. doi: 10.1371/journal.pone.0215713

Fig 1. RanBP9 overexpression does not alter synaptic protein levels in the cortex and hippocampus at 3- months of age.

Fig 1

A: Shows an immunoblotting analysis of rab3A, gap43, drebrin, chromogranin and the house keeping gene actin in brain samples from cortex and hippocampus. Brain homogenates from RanBP9 transgenic (Ran), APΔE9 double transgenic (Dbl), APΔE9/RanBP9 triple transgenic (Tpl) and age-matched wild-type (WT) control mice at 3-months of age were subjected to SDS-PAGE electrophoresis and probed with their respective antibodies. Flag specific monoclonal antibody detected flag-tagged exogenous RanBP9 in the RanBP9 single transgenic and APΔE9/RanBP99 triple transgenic mice only. Actin was used as loading control. The numbers on the left side indicate the molecular weights of each protein. B: Image J quantitation and normalization to actin levels showed no changes in the levels of any of the synaptic proteins at 3 months. The data are mean±SEM, n = 3 for WT and RanBP9 single transgenic, and n = 4 for APΔE9 and APΔE9/RanBP9 genotypes.