Table 6.

Examples of various environments in which Methylocystis were detected among other methanotrophs

Region	Environments/soil type	Methanotrophs	pH	Methods of identification	Literature
Tierra del Fuego, Argentina	Sphagnum bog	Methylocystis, Methylosinus Methylomonas	3.5–4.6	pmoA coloning, sequencing	[45]
Minnesota	Boreal oligotrophic peatland	Methylocystis, Methylomonas, Methylovulum	3.5–4.0	pmoA targeted NGS	[2]
Poland, Lublin region	High moor peat	Methylocystis Methylosinus	4.43	Sequencing of 16S rRNA fragments specific for methanotrophs	[46]
China, Jiangsu	Rice paddy field	Methylococcus Methylocaldum (both type I), and Methylocystis (type II),	Not given	pmoA, T-RFLPa analysis, cloning, sequencing	[47]
Germany	Forest, meadow, pasture	Methylocaldum Methylosinus Methylocystis	4.6–8.0	DGGEbpmoA bands sequencing, PLFA analysis	[25]
European part of Russia	Unmanaged and managed soils	Methylosinus Methylocystis Methylocaldum Methylobacter	4.2–8.2	pmoA coloning, sequenceing	[19]
Nakorn Ratchasrima province, Thailand	Upland different use soils (forest, reforested, cornfields	Methylocystis Methylobacter Cluster 5	4.2–5.6	DGGEb pmoA and mmoX bands reamplified and sequencing	[26]
Russia, Moscow	Forest and agricultural soddy-podzolic soils	Methylocaldum Methylocystis	4.6–5.3	DGGEbpmoA bands cloning and sequencing	[10]
Poland, Jastrzębie-Moszczenica, and Bogdanka coal mines	Coal mine	Methylobacter Methylocaldum Methylosinus Methylocystis	7.56–8.9	pmoA cultured methanotrops, sequencing, FISHc	[48, 49]
Poland, Lublin region	No-tillage and agricultural	Methylocystis	4.78–7.22	bacterial 16S rRNA gene V3-V4 variable region, NGS	This study

^aT-RFLP—terminal restriction fragment length polymorphism analysis

^bDGGE—denaturing gradient gel electrophoresis

^cFISH—fluorescent in situ hybridization