Key resources table.
| Reagent type (species) or resource |
Designation | Source or reference | Identifiers | Additional information |
|---|---|---|---|---|
| Genetic reagent (M. musculus) | C57BL/6J | Jackson Laboratory | RRID: IMSR_JAX:000664 | |
| Genetic reagent (M. musculus) | B6.Tg(Colgalt2-cre)NF107Gsat/Mmucd | Dr. Chip Gerfen (NIH) PMID: 20023653 |
RRID: MGI:2138232 | Referred to as Glt25d2 |
| Genetic reagent (M. musculus) | B6.TgN(grm2-IL2RA/GFP)1kyo | Dr. Robert Duvoisin (OHSU) PMID: 9778244 |
RBRC: RBRC01194 | |
| Genetic reagent (M. musculus) | Ai9(RCL-tdT) | Jackson Laboratory PMID: 22446880 |
RRID: IMSR_JAX:007909 | |
| Genetic reagent (M. musculus) | Ai32(RCL-ChR2(H134R)/EYFP) | Jackson Laboratory PMID: 22446880 |
RRID: IMSR_JAX:024109 | |
| Genetic reagent (M. musculus) | B6.Cg-Et(tTA/mCitrine)TCGOSbn | Dr. Adam Hantman (Janelia Farm) PMID: 26999799 |
||
| Antibody | Chicken polyclonal anti-GFP | Aves Labs | Cat # GFP-1020 RRID: AB_10000240 |
IHC (1:2000) |
| Antibody | Rabbit polyclonal anti-DsRed | Clontech | Cat# 632496 RRID:AB_10013483 |
IHC (1:2000) |
| Antibody | Goat polyclonal anti-mCherry | Sicgen | Cat# AB0040-200 RRID:AB_2333092 |
IHC (1:2000) |
| Antibody | Mouse monoclonal anti-rat mGluR1a | BD Pharmingen | Cat# 556389 RRID:AB_396404 |
IHC (1:800) |
| Antibody | Rabbit polyclonal anti-calretinin |
Swant | Cat# 7697 RRID:AB_2619710 |
IHC (1:500–2000) |
| Antibody | Goat polyclonal anti-calretinin |
Swant | Cat# CG1 RRID:AB_10000342 |
IHC (1:500–2000) |
| Antibody | Rabbit polyclonal anti-shank1 | Synaptic Systems | Cat# 162 013 RRID:AB_2619859 |
IHC (1:1000) |
| Antibody | Mouse monoclonal anti-Myo7A | Dr. Peter Barr-Gillespie (OHSU) | IHC (1:500) | |
| Antibody | Donkey polyclonal anti-chicken Alexa Fluor 488 | Jackson ImmunoResearch Labs | Cat# 703-545-155 RRID:AB_2340375 |
IHC (1:500) |
| Antibody | Donkey polyclonal anti-mouse Alexa Fluor 488 | Jackson ImmunoResearch Labs | Cat# 715-545-150 RRID:AB_2340846 |
IHC (1:500) |
| Antibody | Donkey polyclonal anti-rabbit Cy3 | Jackson ImmunoResearch Labs | Cat# 711-165-152 RRID:AB_2307443 |
IHC (1:500) |
| Antibody | Donkey polyclonal anti-goat Cy3 | Jackson ImmunoResearch Labs | Cat# 705-165-147 RRID:AB_2307351 |
IHC (1:500) |
| Antibody | Donkey polyclonal anti-mouse Alexa Fluor 647 | Jackson ImmunoResearch Labs | Cat# 715-605-151 RRID:AB_2340863 |
IHC (1:500) |
| Antibody | Donkey polyclonal anti-chicken Alexa Fluor 647 | Jackson ImmunoResearch Labs | Cat# 703-605-155 RRID:AB_2340379 |
IHC (1:500) |
| Antibody | Streptavidin-Alexa Fluor 647 | ThermoFisher Scientific | Cat# S21374 RRID:AB_2336066 |
IHC (1:2500) |
| Chemical compound, drug | Alexa Fluor 594 hydrazide sodium salt | ThermoFisher Scientific | Cat# A10438 | |
| Chemical compound, drug | GYKI-53655 | Tocris | Cat # 2555 | |
| Chemical compound, drug | JNJ-16259685 | Tocris | Cat # 2333 | |
| Chemical compound, drug | LY-341495 | Tocris | Cat # 1209 | |
| Chemical compound, drug | (+)-MK-801 hydrogen maleate | Sigma | Cat # M107 | |
| Chemical compound, drug | Strychnine hydrochloride | Sigma | Cat # S8753 | |
| Chemical compound, drug |
SR-95531 hydrobromide | Tocris | Cat # 1262 | |
| Chemical compound, drug | 4-Aminopyradine | Tocris | Cat # 940 | |
| Recombinant DNA reagent | AAV1-CAG-ChR2(H134R)-mCherry (2.92E12 GC/ml) | University of Pennsylvania Vector Core | Cat # CS0949 | |
| Recombinant DNA reagent | AAV9-CAG-ChR2(H134R)-mCherry (2.96E12 GC/ml) | University of Pennsylvania Vector Core | Cat # CS0916 | |
| Recombinant DNA reagent | AAV2-retro-CAG-GFP (1.0E13 GC/ml) | Janeila Farm Vector Core PMID: 27720486 |
Dr. Adam Hantman (Janelia Farm) | |
| Recombinant DNA reagent | AAV2-retro-CAG-tdTomato (7.0E12 GC/ml) | Addgene PMID: 27720486 |
Cat # 59462-AAVrg | |
| Recombinant DNA reagent | AAV2-retro-CAG-Flex-GFP (9.86E12 GC/ml) | Janeila Farm Vector Core PMID: 27720486 |
Dr. Adam Hantman (Janelia Farm) | |
| Recombinant DNA reagent | AAV-PHP.S-CAG-tdTomato (1.7E13 GC/ml) | Addgene PMID: 28671695 |
Cat # 59462-PHP.S | |
| Software, algorithm | pClamp 10 | Molecular Devices | RRID:SCR_011323 | |
| Software, algorithm | Igor Pro 6 | WaveMetrics | RRID:SCR_000325 | |
| Software, algorithm | Prism 7 | GraphPad | RRID:SCR_002798 | |
| Software, algorithm | Excel | Microsoft | RRID:SCR_016137 | |
| Software, algorithm |
Imaris | Bitplane | RRID:SCR_007370 | |
| Software, algorithm | Zen Black | Zeiss | RRID:SCR_013672 | |
| Software, algorithm |
FIJI | https://fiji.sc | RRID:SCR_002285 | |
| Software, algorithm | ImageJ | https://imagej.nih.gov/ij/ | RRID:SCR_003070 | |
| Software, algorithm |
Affinity Designer | Serif | RRID:SCR_016952 |