Fig. 4. Brichos and clusterin exhibit modular and additive behavior of their specific inhibition processes.

(A) Kinetic profiles of 3 μM Aβ(M1-42) solutions in 20 mM sodium phosphate buffer at pH 8.0 in the absence and presence of 18 nM clusterin and 2 μM proSP-C Brichos added either individually or together as indicated at 37°C. (B) The additivity of the inhibition effects reveals that the sites associated with the two different microscopic steps of elongation and secondary nucleation are distinct. Continuous lines represent model simulations where either the elongation rate constant (green line), secondary nucleation rate constant (red line), or both (blue line) have been selectively inhibited.