Table 1.

Genetic Profiling of αT3-1 and LβT2 Cell Lines

A	2-nt Repeat Marker		Fragment Size (bp)
		Chromosome	αT3-1 Cells	LβT2 Cell Stock 1	LβT2 Cell Stock 2	C57BL/6J Mice	BALB/cJ Mice
	4	1	156, 164	156, 170	156, 170	156	160
	5	2	127	113	113	113	127
	136	2	161	149	149	149	160
	78	3	197, 202	202	202	197	202
	134	3	104	111	111	112	104
	14	4	95, 104	95	95	95	105
	94	5	113	113	113	113	111
	16	5	136	136, 143	136, 143	136	143
	139	5	106, 121	106, 121	106, 121	121	106
	144	6	207	208	207	193	211
	25	6	137	137	137	141	137
	133	7	77	82	81	82	78
	138	7	186	191	191	191	182
	163	7	240	219	219	219	242
	27	8	150, 163	163	163	151	165
	39	9	157, 174	157, 174	157, 174	173	157
	165	10	197	197	197	197	191
	141	10	95, 118	95	95	95	114
	74	11	102, 119	102, 119	102, 119	119	102
	111	11	148	148	148	148	144
	20	12	155	155	155	153	155
	31	13	167	167, 198	167	198	167
	137	14	203	204	204	204	209
	143	14	136, 144	132	132	133	137
	53	15	96	96	96	96	82
	171	16	216	210, 216	210, 216	210	216
	47	19	119	119	119	114	119

B	4-nt Repeat Marker		Repeat Number
		Chromosome	αT3-1 Cells	LβT2 Cell Stock 1	LβT2 Cell Stock 2	C57BL/6J Mice	BALB/cJ Mice
	MCA-4-2	4	20.3, 21.3	20.3, 21.3	20.3, 21.3	20.3	21.3
	MCA-5-5	5	14, 17	13, 17	13, 17	17	14
	MCA-6-4	6	18	19	19, 20	18	17
	MCA-6-7	6	12	12	12	17, 18	12
	MCA-9-2	9	15, 18	18	18	18	15
	MCA-12-1	12	16	16, 17	16, 17	17	16
	MCA-15-3a	15	21.3	22.3	22.3	22.3	22.3
	MCA-18-3	18	16, 17	16, 17	16, 17	16	18
	MCA-X-1	X	28, 29	25	25	27	24

The genotypes of the αT3-1 cell line and two LβT2 cell stocks were compared with those of C57BL/6J and BALB/cJ mice. STR profiles were generated using either (Table 1A) a panel of 27 dinucleotide repeat-based markers or (Table 1B) a panel of nine tetranucleotide repeat-based markers. In Table 1A, an allele call is presented as the fragment size (in bp) of a PCR product obtained at a particular locus. Note that a 1-bp difference in fragment size between the αT3-1 sample and one of the comparison profiles represents only run-to-run variability. In Table 1B, an allele call is presented as the number of repeats detected at a particular locus.

^aThe marker is uninformative between mouse strains.