Figure 3.

A chronic HF diet compared with an LF diet does not exacerbate hyperphagia in male or female Pomc^wt/wt or Pomc^tm1/tm1 mice but reduces grams of food eaten by female Pomc^wt/wt and Pomc^tm1/tm1 mice. (A–C) Male food intake. (D–F) Female food intake. (A and D) Grams of food eaten for the first 2 d after diet introduced at weaning for 12 Pomc^wt/wt mice (three cages) and 12 Pomc^tm1/tm1 mice (three cages). Data are shown as mean ± SEM (n = 3) and significant differences were determined using a two-way ANOVA and Sidak post hoc analysis. **P < 0.01; ***P < 0.001. (B and E) Average food intake per cage per day during 35 d is shown for 12 male (three cages) and 12 female (three cages) mice fed either an LF or HF diet. Data are shown as mean ± SEM (n = 3). Significant differences for food intake were determined using a two-way repeated measures ANOVA. F statistics and Sidak post hoc analysis data are shown in an online repository (32). (C and F) Average food intake per cage per day during 16 wk is shown for 12 male (three cages) and 12 female (three cages) mice fed either an LF or HF diet. Data are shown as mean ± SEM (n = 3). Significant differences for food intake were determined using a two-way repeated measures ANOVA. F statistics and Sidak post hoc analysis data are shown in an online repository (32).