TABLE 2.
MICs of compound 2A against strains PAO1 ΔlolCDE::CTX-lolCDEPAO1 and PAO1 ΔlolCDE::CTX-lolCDEE. coli and derivativesa
| Strain modification | MIC (µg/ml) of compound 2A for: |
|
|---|---|---|
| PAO1 ΔlolCDE::CTX-CDEPAO1 | PAO1 ΔlolCDE::CTX-CDEE. coli | |
| None | >64 | >64 |
| ΔmexAB-oprM | >64 | 16 |
| + FpvA-ΔP | >64 | 16 |
| ΔmexAB-oprM + FpvA-ΔP | >64 | 4 |
The Pseudomonas PAO1 strains with the E. coli and P. aeruginosa LolCDE systems were grown in the presence of arabinose. FpvA-ΔP expression from plasmid pMMB67EH-fpvA-ΔP was induced with 200 µM IPTG.