Figure 6.

Viability for an 8 × 10⁵ cell/mL cell density encapsulated in a 0.8 mg/mL collagen hydrogel remains minimally disturbed after chemical studies performed after the stimulation with 5 mM CaCl₂, 1 mM glutamate, 1 μM ionomycin. Incorporation of BAPTA-AM did not cause detrimental effects, and cell remained viable after the incorporation of Ca²⁺ (Left). All images and viability test were acquired after the incubation (> 1 min) with all triggers. Green and red fluorescent (viable and dead, respectively) images when acquired with a p-value of 0.945 indicate there is no significant difference between the cell viabilities with stimulus and the control.