Figure 8.
The ATP E-AB sensor exhibited no appreciable response to catecholamine release from PC12 cells cultured in the sensor 3D cell culture array. (Top left) Stimulation with Ca2+ shows baseline sensor response (light red), comparable to electrochemical recordings when no stimulus is added to the cell culture media (black line trace). (Top right and bottom leftSimilarly, the sensor did not respond to catecholamine release upon the addition of glutamate and ionomycin (traces). comparison of the signal change generated by astrocytic Ca2+, glutamate and ionomycin-triggered ATP release (dark red, purple and orange time traces, respectively) confirms the specificity of the E-AB sensor to extracellular ATP presence after same stimuli were exerted in astrocytes cultured in same conditions. These figures encompass adjacent averaged (n=5) raw data for the E-AB sensor response. (Bottom right) Control viability studies helped to visualize the stability of PC12 cells under current experimental conditions. Fluorescent images were acquired with a p-value of 0.183 indicating there is no significant difference between the cell viabilities with stimulus and the control.