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Kinetic curves for the catalytic oxidation of Fe(II) by recombinant human homopolymer apo-HuHF (left), recombinant human heteropolymer apo-HL (middle), and apo-HosF (right). The experimental conditions are: HuHF (0.5 μM), HL (0.5 μM), HosF (5 μM), in 100 mM MOPS buffer and 100 mM NaCl, pH 7.4 and 25 °C. The ratio of Fe(II)/protein is shown on each panel and is color coded.
