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. 2018 Sep 12;27(1):129–137. doi: 10.1007/s10787-018-0528-3

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© The Author(s) 2018

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.

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Fig. 4 — Representative immunoblot showing the expression of a IL-1β; b IL-6; c TNF-α protein in asymptomatic (4 d.p.i.) and symptomatic (12 d.p.i.) phases of the disease in brain homogenates of control and immunized rats treated or not with BBG. The graph indicates the results of densitometric measurements of 3–4 independent immunoblots performed using 3–4 distinct forebrains and expressed as a percentage of control. The relative density was calculated against actin as an internal standard. *p < 0.05, **p < 0.01 and ***p < 0.001 vs. EAE group or control non-immunized group (one-way ANOVA with the post hoc Dunnett’s test)