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. 2019 Apr 17;10:1796. doi: 10.1038/s41467-019-09746-1

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 1 — Stat3 promotes mitochondrial function in muscle stem cells (MuSCs) during activation. a Scheme of the experimental design to obtain the samples for RNA-seq. b Heat map of the differentially expressed genes among the different comparisons. Listed genes were differentially expressed in at least one of the comparisons. Normalized gene RPKM (reads per kilobase of transcript per million) values were averaged within groups for the generation of the heat map (n = 3 animals). c Pie charts showing the top 20 differentially enriched pathways (using Gene Set Enrichment Analysis (GSEA)) during the activation of control (Pax7-CreER^WT;Stat3^f/f) or Stat3 KO MuSCs (Pax7-CreER;Stat3^f/f mice) grouped according to their functional category. d Top 12 pathways enriched in freshly isolated MuSCs from 3 dpi Pax7-CreER;Stat3^f/f mice compared to 3 dpi MuSCs isolated from control littermates using GSEA. The red line indicates p value = 0.05 based on the pathway analysis (GSEA). e–g Measurement of the oxygen consumption rate (OCR) and the extracellular acidification rate (ECAR) of control and Stat3 KO MuSCs cultured in growth conditions for 3 days (n = 4 independent experiments). The Cell Mito Stress Test was performed in a Seahorse XFp Analyzer. Data are represented as mean ± SEM (Student’s t test or two-way analysis of variance; *p < 0.05, ***p < 0.001, ****p < 0.0001)