Fig. 4.

Fam3a ablation compromises skeletal muscle development in vivo. a Scheme of the generation of the Fam3a knockout (KO) mouse model and the experimental design. b–d Immunofluorescence analysis of transversal sections of the hind limbs of wild-type (WT) and Fam3a KO E15 embryos. Quantification of the embryonic myosin heavy chain (eMyHC) area and the number of Pax7- and Myogenin-positive cells (n = 5 embryos). Scale bars 300 and 30 μm. e, f Immunofluorescence analysis of transversal sections of the hind limbs of WT and Fam3a KO P0 pups. Quantification of the eMyHC area and the number of Pax7-positive cells (n = 3–4 pups). Scale bars 100 and 25 μm. Data are represented as mean ± SEM (Student’s t test; *p < 0.05, **p < 0.01)