Figure 2.

Collapse and re-expansion of blastocysts. (A) The proportion of blastocysts that spontaneously collapsed after 72 h of storage at different temperatures and in different medium supplementations and the re-expansion ability of the collapsed blastocysts after 24 h of conventional culture (NCSU-23 culture medium supplemented with 0.4% BSA and 10% FCS at 38.5 °C in humidified air with 5% CO₂). ^a,bDifferent letters shown per variable (collapsed or reformed) indicate significant differences (P < 0.05). BL: blastocyst. (B) Representative pictures of blastocyst collapse and re-expansion. (a) blastocysts just after collection; (b) selected blastocysts collapsed after 72 h of storage; (c) re-expansion of blastocysts after 24 upon conventional culture. Scale bar is 100 µm.