. 2019 Apr 17;9:6219. doi: 10.1038/s41598-019-42712-x

Table 1.

Survival rates of in vivo-produced porcine morulae after 72 h of storage at different temperatures and in different medium supplementations.

Group	Storage temperature	Number of embryos	72 h of storage [Number of embryos (%)]		48 h of conventional culture [Number of embryos (%)]^#
Group	Storage temperature	Number of embryos	Viable	Degenerated	Viable	Degenerated	Cell numbers per blastocyst
50%-FCS	17 °C	21	11 (52.4)^a	10 (47.6)^a	8 (38.1)^a	13 (61.9)^a	63.8 ± 6.8^a
4%-BSA	17 °C	22	13 (59.1)^a	9 (40.9)^a	10 (45.4)^a	12 (55.6)^a	64.5 ± 13.1^a
50%-FCS	20 °C	48	48 (100)^b	0 (0.0)^b	30 (62.5)^a	18 (37.5)^a	61.4 ± 9.9^a
4%-BSA	20 °C	45	44 (97.8)^b	1 (2.2)^b	37 (82.2)^b	8 (17.8)^b	70.1 ± 9.2^a
Control^##		41	—	—	40 (97.6)^c	1 (2.4)^c	114.4 ± 19.8^b

^#After storage, the viable stored embryos from each group were`ultured for an additional 48 h under conventional culture conditions (NCSU-23 culture medium supplemented with 0.4% BSA and 10% FCS, at 38.5 °C in humidified air with 5% CO₂) to reassess their in vitro survival. ^##Control morulae were cultured under conventional conditions for 48 h. ^a,b,cDifferent superscripts in the same column represent significant differences (P < 0.05).