. 2019 Apr 17;9:6219. doi: 10.1038/s41598-019-42712-x

Table 3.

Survival rates of in vivo-produced porcine blastocysts after 72 h of storage at different temperatures and in different medium supplementations.

Group	Storage temperature	Number of embryos	End of storage [Number of embryos (%)]			24 h of conventional culture [Number of embryos (%)]^#
Group	Storage temperature	Number of embryos	Viable	Collapsed	Degenerated	Viable	Collapsed	Degenerated	Cell numbers per blastocyst
50%-FCS	17 °C	29	6 (20.7)^a	2 (6.9)^a	21 (72.4)^a	7 (24.1)^a	1 (3.4)	21 (72.4)^a	86.4 ± 9.0^a
4%-BSA	17 °C	31	5 (16.1)^a	8 (25.8)^a	18 (58.1)^a	13 (41.9)^a	0 (0.0)	18 (58.1)^a	90.3 ± 12.2^a
50%-FCS	20 °C	55	27 (49.1)^b	27 (49.1)^b	1 (1.8)^b	40 (72.7)^b	1 (1.8)	14 (25.4)^b	90.1 ± 17.3^a
4%-BSA	20 °C	60	27 (45.0)^b	29 (48.3)^b	4 (6.7)^b	48 (80.0)^b	0 (0.0)	112 (20.0)^b	92.9 ± 13.3^a
Control^##		49	—	—	—	48 (97.9)^c	0 (0.0)	1 (2.1)^c	131.2 ± 11.9^b

^#After storage, the viable stored embryos from each group were cultured for an additional 24 h under conventional culture conditions (NCSU-23 culture medium supplemented with 0.4% BSA and 10% FCS, at 38.5 °C in humidified air with 5% CO₂) to reassess their in vitro survival. ^##Control blastocysts were cultured under conventional conditions for 24 h. ^a,b,cDifferent superscripts in the same column represent significant differences (P < 0.05).