Fig. 2.

Single-molecule electro-optical detection of 5 kbp DNA labelled with YOYO-1. a Photon and current time traces for the translocation of 100 pM 5 kbp DNA-YOYO-1 in 100 mM KCl, 10 mM Tris-HCl, 1 mM ethylenediaminetetraacetic acid (EDTA) buffer (pH = 8). The resampling time for the photon time trace is 500 μs and the filter cut-off frequency for the current time trace is 10 kHz. Laser power was 90 ± 3 μW. b Percent synchronisation, c signal to noise and d dwell time as a function of applied voltage for the electrical (blue circles) and optical (brown squares) channels respectively. The error bars represent the accumulation of statistics from at least three different nanopipettes