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. 2019 Apr 17;9:6225. doi: 10.1038/s41598-019-42522-1

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Secretion of apoE in the human perfused placenta. Four term human placentas were initially perfused for 30 minutes with DMEM medium and EBSS buffer containing 2 g/L of glucose, 10 g/L Dextran FP40, 40 g/L BSA and 2.5 IE/mL heparin. This washing phase was followed by 240 minutes perfusion of the maternal and fetal circulation as an open/open system. Samples were taken at 15, 30, 90, 120, 150, 180, 210 and 240 minutes. (A,B) The concentration of apoE in the maternal (A) and the fetal perfusate (B) was measured at each time point by ELISA and normalized to the weight of the cotyledon (g). (C) Measurement of apoE concentrations in placental tissue lysates before and after the perfusion by ELISA. The concentration was normalized to the total protein content (mg). Results represent mean ± SEM of four independent experiments measured in triplicates; *p < 0.05.