Figure 4.

Secretion of apoA1 and apoE by primary hepatocytes and primary trophoblasts. Primary hepatocytes and primary trophoblast cells were isolated from human liver of colorectal metastases patients (n = 6) and from healthy human term placentas (n = 6) as described in the Materials and Methods section. Primary hepatocytes and primary trophoblasts were seeded at a density of 75000 cells/cm² and 0.5 × 10⁶ cells/cm², respectively, on conventional plates and cultured for three days. Each day, the medium was collected and cellular protein content determined. (A,B) Quantification of apoA1 (A) and apoE (B) by ELISA. The concentration was normalized to the respective protein concentration. Results represent mean ± SEM of six independent experiments performed in triplicates for primary hepatocytes and primary trophoblasts, respectively; **p < 0.01; ***p < 0.001.