Figure 3.

LMO4 is overexpressed in several human breast cancer cell lines. Northern blot analysis of poly(A)⁺ RNA (3 μg) from human and mouse (SCp2) breast epithelial cell lines. Filters were hybridized sequentially with mouse Lmo4, Ldb1, and Gapdh cDNA probes. The lower molecular weight LDB1 transcript in human breast cancer cell lines may represent a cross-hybridizing species. Sizes of the Lmo4 and Ldb1 transcripts were lower in the mouse SCp2 cell line relative to those in human cell lines. GAPDH, glyceraldehyde-3-phosphate dehydrogenase.