Figure 5.

Overexpression of LMO4 RNA and LMO4 protein in primary breast cancer. In situ hybridization (with digoxigenin-labeled human LMO4 riboprobes) and immunohistochemistry (with a rat anti-LMO4 monoclonal antibody) were performed on tissue arrays containing archival breast specimens. High LMO4 RNA expression in an infiltrating lobular carcinoma (A), and two infiltrating ductal carcinomas (B and C) (antisense LMO4 probe); (D) benign fibroadenoma, displaying low expression of LMO4 RNA. Abundant LMO4 protein expression was detected in the corresponding infiltrating lobular and ductal cancer samples (E–G). Low levels of LMO4 protein were detected in the benign sample (H). Corresponding negative controls (Ig) for immunostaining are shown (I–K); (L) ductal carcinoma in situ, showing high expression of LMO4 RNA. Sense LMO4 probe gave no signal for any of the tumor samples. (Original magnification: A–D, ×100; E–K, ×200; L, ×50.)