Figure 7.

Cell-mediated immune responses in vaccinated mice. C57BL/6 mice were immunized according to schedule and procedures as in Materials and Methods. IFN-gamma production was measured in an ELISPOT assay after specific antigenic stimulation with the E7 peptide RAHYNIVTF (aa 49–57). A, empty pVax prime/mock hairy root extract boost; B, mock hairy root extract prime/mock hairy root extract boost; C, pVax-E7*-SAPKQ prime/pVax-E7*-SAPKQ boost; D, pVax-E7*-SAPKQ prime/E7*-SAPKQ extract boost; E, SAPKQ extract prime/SAPKQ extract boost. Data are presented as fold-increase responses to the E7 peptide in comparison with mice vaccinated with empty vector/irrelevant root extract, and they represent the means of all of the mice in the groups ± SD.