TABLE 1.
Bacterial strains and plasmids used in this study
| Strain or plasmid | Relevant characteristics | Source or reference |
|---|---|---|
| Strains | ||
| Escherichia coli | ||
| DH5α | supE44 ΔlacU169 (φ80 lacZΔM15) hsdR17 recA1 endA1 gyrA96 thi-1 relA1 | 67 |
| BL21(DE3) | F−- ompT gal dcm lon hsdSB(r− m−; E. coli B strain), with DE3, a λ prophage carrying the T7 RNA pol gene | Stratagene |
| Pseudomonas aeruginosa | ||
| PAO1 | Reference strain | 68 |
| Salmonella enterica serovar Typhimuriuma | ||
| 14028 | Wild type | ATCC |
| SV4338 | aroA551::Tn10 | Laboratory stock |
| SV5136 | ssaV::Cm | Laboratory stock |
| SV8462 | ΔaroB::Km | Laboratory stock |
| SV9699 | aroA551::Tn10 ΔaroB::Km | This work |
| Plasmids | ||
| pET15b | 6×His fusion vector, Apr | Novagen |
| pET15b-OprF/I | OprF/I cloned with NdeI and BamHI | This work |
| pGEX-4T-2 | GST fusion vector, Apr | GE Healthcare |
| pIZ2160 | pWSK29-PsseA-SseJ-OprF/I-FLAG | This work |
| pIZ2267 | pWSK29-PsseA-SseJ-PcrV-FLAG | This work |
| pIZ2338 | pGEX-4T-2-PcrV | This work |
| pWSK29 | Low-copy-number vector, Apr | 50 |
a
Derivatives of these strains were used as indicated in the text.