TABLE 2.

Oligonucleotides used in this study

Oligonucleotide	Sequence 5′→3′
Amplification of pcrV with FLAG tag for cloning in pWSK29
pcrVfwBam	ATCGGGATCCGAAGTCAGAAACCTTAATGC
pcrVFLAGrevNot	GCATGCGGCCGCCTATTTATCGTCGTCATCTTTGTAGTCGATCGCGCTGAGAATGTCGC
Fusion of oprF and oprI with FLAG tag
oprFfwBam	ATCGGGATCCGCTCCGGCTCCGGAACCGGTTGCCGAC
oprFrev	TTCAACGCGACGGTTGATAGCGCG
oprF/oprIfw	GAAGGCCGCGCTATCAACCGTCGCGTTGAAAGCAGCCACTCCAAAGAAAC CGAAGCT
oprIFLAGrevNot	GCATGCGGCCGCCTATTTATCGTCGTCATCTTTGTAGTCCTTGCGGCTGGCTTTTTCCAG
Amplification of sseJ
sseJfwEco	CCTAGAATTCGTAAGGAGGACACTATGCC
sseJrevBam	ACGTGGATCCTTCAGTGGAATAATGATGAG
Amplification of sseA promoter
PsseAfwKpn	GCTAGGTACCAGAAGAGAACAACGGCAAG
PsseArevEco	CACTGAATTCACGATAGATAATTAACGTGC
Amplification of pcrV for cloning in pGEX-4T-2
pcrVfwBam	ATCGGGATCCGAAGTCAGAAACCTTAATGC
pcrVrevEco	ATCGGAATTCCTAGATCGCGCTGAGAATGT