TABLE 2.

Opaque cells of a/α sfl2Δ mutants do not mate^a

Strains crossedb	Mating period (no. of days)	Mating frequencyc
P37005-Hr (a/a) × WO-1-Sr (α/α)	2	(4.0 ± 0.2) × 10−3
	4	(6.7 ± 0.4) × 10−3
P37005-Sr (a/a) × SC5314sfl2Δ-Hr (a/α)	2	(<1.3 ± 0.1) × 10−8
	4	(2.8 ± 3.5) × 10−7
P37005-Sr (a/a) × P37039sfl2Δ-Hr (a/α)	2	(<1.3 ± 0.2) × 10−8
	4	(<8.7 ± 1.6) × 10−9
WO-1-Sr (α/α) × SC5314sfl2Δ-Hr (a/α)	2	(<1.1 ± 0.1) × 10−8
	4	(3.5 ± 2.3) × 10−7
WO-1-Sr (α/α) × P37039sfl2Δ-Hr (a/α)	2	(<1.1 ± 0.1) × 10−8
	4	(<9.5 ± 2.0) × 10−9

^aMating was tested on GlcNAc agar at 25°C in air. Mating was assessed as the frequencies of offspring in the population resistant to both hygromycin B and nourseothricin. Descriptions of the strains generated for mating are presented in Table S1 in the supplemental material. At the end of the strain names, Hr and Sr stand for hygromycin B resistant and nourseothricin resistant, respectively.

^bAll cells tested were in the opaque phase.

^cFrequencies are presented as means ± standard deviations.