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. 2019 Apr 17;4(2):e00154-19. doi: 10.1128/mSphereDirect.00154-19

FIG 1.

FIG 1

Experimental procedure for the isolation of bacterial RNA from infected tissue. Infected lungs were homogenized with a Dounce glass homogenizer, and the lung homogenate was filtered through a 70-μm-pore-size cell strainer to remove tissue debris. The strained homogenate was pushed through a 5-μm-pore-size syringe filter to exclude eukaryotic cells and capture the bacteria in the filtrate. The captured bacteria were pelleted by centrifugation, and the contaminating murine RNA (due to cell lysis) was removed. Once supernatant containing contaminating RNA was removed, the bacterial pellet was resuspended in RNAprotect to stabilize the bacterial cells until RNAsnap isolation.