Fig. 3.

One-step recombinant bacterial STs-based labeling of glycans in tissue specimens. The embryonic frozen sections from E16 mouse were incubated with STs (Pm2,3ST-M144D or Pd2,6ST) or without STs, and CMP-SiaNAz-biotin, followed by Alexa Fluor 594-streptavidin conjugate staining. The resulting fluorescence (red) of different parts of the embryo was directly imaged using microscopy, including salivary glands region, lateral sections of spine, and anterior chest. The cells of frozen sections were stained with anti-actin (green) and DAPI (blue, nucleus). Scale bar, 1 mm