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. 2019 Apr 17;10:1799. doi: 10.1038/s41467-019-09608-w

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Fig. 4 — A cell-based glycan array to assess HA–glycan interactions directly on live cells. a Profiling glycoforms of lung tissues obtained from healthy human donors. Lung tissue slides were stained with FITC-AAL, AF647-anti-CLA, Biotin-MAA, or Biotin-SNA conjugates to detect α1-3-fucosylation, sLeX epitopes, α2-3-linked, or α2-6-linked sialylation, respectively. b Major glycan epitopes presented on Lec2 cell-surface after chemoenzymatic glycan modification. CHO Lec2 cells were treated with glycosyltransferases indicted above and the corresponding nucleotide sugars. *indicates the potential modification site for the first-step glycan modification (black), and the second-step glycan modification (gray). c Relative binding affinity of HA from HK68 (H3N2) for glycan-modified Lec2 cells using the specified recombinant glycosyltransferases. In Fig. 4c, the error bars represent the standard deviation of six biological replicates. Source data for figure c are provided as a Source Data file