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HPLC analysis of products by displayed pSIase. Trehalulose (T), Isomaltulose (I). The effect of reaction temperatures on products was tested by 10 ml wet cells solution harboring pSIase were mixed with 100 ml 20% (m/v) sucrose solutions as a substrate at 40 °C (a) and 30 °C (b) for 2 h
