Fig. 2.

Steps in in planta process of Agrobacterium-mediated transformation. a, b Seedlings germinated after 2 days on soaked germination paper. c Injection with Agrobacterium culture (having pCAM1301-Ubi-CP4-EPSPS construct) on shoot tip. d Seedlings kept for co-cultivation in sterile soilrite. e, f Fully grown T₀ wheat plants transferred to pots till maturity. g T₁ plants obtained after seed germination. h Survived plants after glyphosate spraying; the black marked region represents the WT plants or negative control (HD2894). i Putative transgenics transferred to pots for further germination