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. 2019 Apr 17;9:6201. doi: 10.1038/s41598-019-42657-1

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Effects of vehicle, Ang II and Ang II + RKT treatments on renal histopathological changes. (A) Representative images of PAS-stained kidney sections in vehicle, Ang II and Ang II + RKT groups (original magnification, ×200; bar, 100 μm). (B) Representative images of MT- and an anti-type 3 collagen antibody-stained consecutive kidney sections in vehicle, Ang II and Ang II + RKT groups (original magnification, ×400; bar, 100 μm; asterisk, enhanced region of the anti-type 3 collagen antibody). (C) Representative images of an anti-F4/80 antibody-stained kidney sections in vehicle, Ang II and Ang II + RKT groups (original magnification, ×400; bar, 100 μm; arrow, F4/80 positive cell). Ang II, angiotensin II; RKT, rikkunshito; PAS, periodic acid-schiff; MT, masson trichrome.