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. 2019 Apr 17;9:6201. doi: 10.1038/s41598-019-42657-1

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Effects of vehicle, Ang II and Ang II + RKT treatments on renal ghrelin signaling pathway. (A) Renal mRNA expression of ghrelin receptor in vehicle, Ang II and Ang II + RKT groups (n = 6–7). (B) Renal mRNA expression of sirtuin 1 in vehicle, Ang II and Ang II + RKT groups (n = 6–7). (C) Renal mRNA expression of GLP-1 receptor in vehicle, Ang II and Ang II + RKT groups (n = 6–7). (D) Representative western blots and quantitative analysis of renal GLP-1 protein levels in vehicle, Ang II and Ang II + RKT groups (n = 6–7). *P < 0.05, vs vehicle group. Data were analyzed by one-way ANOVA. Ang II, angiotensin II; RKT, rikkunshito; GLP-1, glucagon-like peptide-1.