FIGURE 1.

General Interactomic Workflow. To accurately map the interactome of a protein of interest (POI), begin by selecting your experimental method (Screen/Precipitate). To improve the recovery of low abundance proteins and to validate possible novel binding partners it is strongly suggested to repeat the assay two to four times then compile the results prior to filtering. To define the true-positives from false-positives, use a filtering process (Filter) followed by a ranking process (Rank) to generate a list of high confidence candidates. After ranking, it is equally acceptable to proceed directly to the data analysis step or to first confirm the previous results either by repeating the precipitation then using a different detection method (e.g., LC-MS/MS vs. western blot) or to use a new precipitation method (BioID vs. AP-MS).