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. 2019 Apr 11;9:211. doi: 10.3389/fonc.2019.00211

Figure 4.

Figure 4

HDGF controls cell cycle and EMT-associated gene expression in EC via the PI3K/AKT and β-catenin pathways. Knocking down endogenous HDGF expression reduced the expressions of pRb, E2F1, c-Myc, CDK4, and CCND1, and enhanced P27 in EC cells (A). Suppressing HDGF expression decreased the expression of EMT marker genes (N-cadherin, Vimentin, and Snail) and enhanced E-cadherin expression (B). Reduced HDGF expression decreased the expressions of p-PI3K, p-AKT, and β-catenin (C). Knocking down of HDGF suppressed both nuclear and cytosol protein expression of β-catenin (D). Suppressing the expression of p-PI3K by its specific inhibitor Ly294002 (50 nM) reduced p-AKT, β-catenin, Snail, N-cadherin, Vimentin, and upregulated E-cadherin in overexpressing HDGF EC cells (E,F). β-actin served as the internal control. Each experiment was repeated three times with similar results, and error bars represent the mean ± SD, *P < 0.05, **P < 0.01, ***P < 0.001.