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. 2019 Apr 11;9:211. doi: 10.3389/fonc.2019.00211

Figure 6.

Figure 6

Transient overexpression of DDX5 from a plasmid reverses the suppression of shHDGF. Efficiency of plasmid overexpression of DDX5 in EC cell lines (A). Transient increase in the expression of DDX5 by plasmid promotes cell proliferation in EC cells, as assessed by the MTT (B) and EdU incorporation assays (C). Transient upregulation of DDX5 dramatically increases the migration and invasion ability of EC cells in vitro (D). Western blotting analysis of the protein levels of p-PI3K, p-AKT, β-catenin, pRb, E2F1, c-Myc, CDK4, CCND1, and P27, as well as invasion and migration according to the relevant protein levels of E-cadherin, N-cadherin, Vimentin, and Snail after transient transfection of DDX5 plasmid into EC cells (E–G). β-actin served as the internal control. Data are presented as the mean ± SD for three independent experiments (*P < 0.05, **P < 0.01, ***P < 0.001).