Figure 1.

Time line of scaffold-free PDLC construct formation. (A) Micrograph of PDLCs after attaching to construct dishes. (B) Micrograph of PDLCs reaching confluence on construct dishes approximately 4–5 days following cell plating. (C) Image of PDLC sheet contracting from sides of well of six-well plates toward two pins placed in the center of dish; green arrows are pointing to pins. (D) Higher magnification micrograph of green boxed area is shown in (C). (E) Image of final, cylindrical scaffold-free PDLC construct anchored to the dish on either end by pins. Scale bars: (A,B,D) = 200 μm, (C): 10 mm, and (E): 5 mm.