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. 2019 Apr 11;10:422. doi: 10.3389/fphys.2019.00422

Figure 5.

Figure 5

Immunofluorescent staining of scaffold-free PDLC constructs after 4-week in vivo implantation. (A) PLAP-1 (red) is expressed strongly on the periphery of the scaffold-free constructs; solid white line outlines engineered tissue. (Aʹ) Higher magnification image of dashed boxed region shown in (A). (B) Faint ALP (red) expression can be seen localized in the center of the scaffold-free constructs after implantation; solid white line outlines construct. (Bʹ) Higher magnification image of dashed boxed region shown in (B). (C) Periostin (green) is expressed on the periphery tissue of scaffold-free construct following implantation. (Cʹ) Higher magnification image of boxed region in (C). (D) BSP (red) is expressed in the center tissue of scaffold-free construct following implantation. (Dʹ) Higher magnification image of boxed region in (D). (E) Merged image of (C) and (D) further validates that periostin (green) is expressed on periphery tissue and BSP (red) is expressed in the center tissue of engineered construct. (Eʹ) Higher magnification of boxed region shown in (E). In all images, DAPI was used to stain cell nuclei (blue). Scale bars: (A–E) = 100 μm; (Aʹ–Eʹ) = 50 μm.