Figure 3.

Effects of WHS on the activator protein 1 (AP-1), signal transduction and activation of transcription 1 (STAT1), and mitogen-activated protein kinase (MAPK) signaling pathways in UVB-exposed Hs68 fibroblasts. Cells were irradiated with UVB (15 mJ/cm²) and then treated with WHS (6.25, 12.5, or 25 μg/mL) for 4 h (AP-1) or 30 min (STAT1 and MAPK). (A) Nuclear or (B,C) total cellular protein was prepared and resolved by SDS-PAGE, transferred to PVDF membranes, and detected with specific p-c-Fos (Ser32), p-c-Jun (Ser63), p-STAT1 (S727), p-p38, p-JNK, and p-ERK antibodies. Histone H3 and β-actin were used as internal controls for nuclear and whole-cell lysates, respectively. Presented data are the representative blots of three independent experiments.