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. 2019 Mar 20;20(6):1408. doi: 10.3390/ijms20061408

Figure 1.

Figure 1

Netrin-1 expression and secretion do not change in healthy versus GDM WJ-MSC. (A) Scheme of the experimental procedures. WJ-MSC primary cultures were maintained for 10 days at the indicated glucose concentration in 10% FBS and then incubated for up to 2 more days in either 5 mM or 25 mM plus 2% FBS. Samples were collected at indicated times for analysis. The vertical arrow indicates initiation of differential d-glucose treatment. (B) Whole cell lysates were evaluated by Western blot for Netrin-1 expression in WJ-MSC from healthy and GDM cultures. VEGF was used as a positive control and β-actin was used as internal loading control. Exposure to different d-glucose concentration as indicated. (C) Data represent the mean of independent biological replicates ± SEM (healthy n = 12, GDM n = 6). (D) Quantitation of Netrin-1 by Elisa analysis. CM from healthy and GDM WJ-MSC was obtained to evaluate Netrin-1, CM was obtained with different d-glucose treatment at indicated time points (n = 4).