. 2019 Mar 19;10(3):234. doi: 10.3390/genes10030234

Table 1.

List of primers used for DNA analysis. The table includes primer specificity, marker region, annealing temperature (Ta) based on experimental analysis, amplicon length (bp), and references.

Primer Name	5′-3′	Species Specificity	Marker Region	Ta (Annealing Temperature)	Amplicon Length (bp)	References
Bras_matK F	CTACGCAAGCAGTCTTCTCATT	Brassicaceae	matK	60 °C	280	This study
Bras_matK R	TTGCGATTGAAACCATACGGA	Brassicaceae	matK	60 °C	280	This study
S.alba_ITS2 F	CAGAATCCCGTGAACCATCGAGTC	Sinapis alba	ITS2	64 °C	220	This study
S.alba_ITS2 R	GACAATATGACGAGGTTACAA	Sinapis alba	ITS2	64 °C	220	This study
A6 F	CCAGCGAAGGATTTGACGAC	Brassica juncea, Brassica napus	Bra019579	63 °C	220	[20]
A6 R	GACGAATCGAGTGCCCTG	Brassica juncea, Brassica napus	Bra019579	63 °C	220	[20]
B F	GGCATCTGAAGAGAGAGTCCCTTTG	Brassica juncea, Brassica nigra	pBNBH35	63 °C	280	[21]
B R	ATCTTCTTCTTGCCATGAGTGGCC	Brassica juncea, Brassica nigra	pBNBH35	63 °C	280	[21]
C1 F	TGCTGCGCCGAACAATAG	Brassica napus	Bo1g016520	60 °C	130	[20]
C1 R	CCGATCGTGGTTCATATTGC	Brassica napus	Bo1g016520	60 °C	130	[20]