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. 2019 Mar 25;11(3):700. doi: 10.3390/nu11030700

Figure 4.

Figure 4

Mitochondrial membrane potential [DeltaPsi(m)] of lipopolysaccharide (LPS)-challenged neural cells (with or without curcumin treatment), as determined using JC-1 staining and flow cytometry with or without CISD2 knockdown. Cells presenting a decrease in DeltaPsi(m) and impending cellular apoptosis are indicated by monomer green JC-1 fluorescence, compared to normal cells with intact DeltaPsi(m) (indicated by j-aggregated red fluorescence). All groups of cells were evaluated in terms of the ratio of PE+ to PE−. (A) Representative results of flow cytometry for the following groups of cells: (i) scramble RNA-transfected control cells untreated with LPS; (ii) scramble RNA-transfected, LPS-challenged cells, untreated with curcumin; (iii) scramble RNA-transfected, LPS-challenged cells following curcumin treatment; (iv) siCID2-transfected control cells without LPS or curcumin treatment; (v) siCID2-transfected, LPS-challenged cells without curcumin treatment; (vi) siCID2-transfected, LPS-challenged cells, treated with curcumin. (B) Bars indicate the mean ± SEM (n = 3). #,& p < 0.05, ** p < 0.01, and ###,&&&,*** p < 0.001 indicate differences of statistical significance.