Figure 2.
Effects of KRX-C7 on glucose extraction and GRK2 expression in L6 myoblasts. (A) Effects of KRX-C7 on basal and insulin (Ins) -induced glucose uptake determined by 3[H]-2-Deoxyglucose (2-DOG). Results are expressed as mean ± SEM of three independent experiments. * p < 0.05 vs. Ctr. (B) Effects of KRX-C7 on glucose transporter 4 (Glut4) expression. Densitometry of GLUT4 was corrected for actin. (C) Effects of KRX-C7 on Glut4 membrane translocation. Densitometry of GLUT4 was corrected for Gαs. (D) Effects of KRX-C7 on insulin induced on GRK2 expression in L6 myoblasts. (E) Effects of KRX-C7 on insulin induced on GRK2 translocation to cell membranes in L6 myoblasts. For all graphs, densitometric analysis is expressed after normalization for appropriate control protein. Data points represent the mean ± SEM of three independent experiments. * p < 0.05 vs. Ins−; # p < 0.05 vs. Ins+.