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. 2019 Mar 25;20(6):1492. doi: 10.3390/ijms20061492

Figure 3.

Figure 3

KRX-C7 ameliorates insulin signaling in L6 myoblasts. (A) Effects of KRX-C7 on insulin mediated IRS1 phosphorylation in L6 myoblasts. IRS1 phosphorylation densitometry was corrected by total IRS1 densitometry. (B–D) Total cellular samples were analyzed by WB for Phospho-Ser473AKT, Phospho-tyrosine 42/44 ERK, and Phospho-Ser272-AMPK with specific antibodies. Intensity on WB was quantified by densitometric analysis. Phosphorylation densitometry was corrected by the corresponding total protein densitometry. Data points in all graphs represent the mean ± SEM of three independent experiments. * p < 0.05 vs. Ins−; # p < 0.05 vs. Ins+.