Skip to main content
. 2019 Mar 25;20(6):1492. doi: 10.3390/ijms20061492

Figure 7.

Figure 7

GRK2 inhibition reduces oxidative and hypertrophy responses in the diabetic heart. (A) Western blot analysis of phospho-NF-κB and total NF-κB levels on whole protein lysate from the cardiac tissue of KRX-C7 treated and control db/db mice (* p < 0.05). (B) Gene expression analysis of proinflammatory factors, IL-6, MCP-2, and MMP9, by real-time PCR on total RNA extract from the myocardium of KRX-C7 treated and control db/db mice expressed as fold change of control (* p < 0.05). (C) Levels of protein oxidation in cardiac tissue from KRX-C7 treated and control db/db mice shown as levels of 2,4-dinitrophenylhydrazine (DNP) incorporated into proteins. GAPDH was used as the loading control. The image is representative of three independent experiments. * p < 0.05. (D) Gene expression levels of ANF and SERCa2A, performed by real-time PCR on total RNA extract from the myocardium of C57Bl6 mice, db/db mice, and KRX-C7 treated db/db mice, expressed as fold of C57Bl6 (# p < 0.05 vs. C57Bl6, * p < 0.05 vs. db/db).