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. 2019 Feb 26;11(3):485. doi: 10.3390/nu11030485

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© 2019 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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Expression of some iron regulators and transporters after treated with 25 μM of different iron sources for two hours. Values not sharing a common letter differ significantly (p < 0.05). W: wild-type cells treated with 0 μM iron; W25S, W25G: wild-type cells treated with 25 μM FeSO₄, 25 μM Fe-Gly; K0: DMT1-knockout cells treated with 0 μM iron; K25S, K25G: W0: wild-type cells treated with 0 μM iron; W25S, W25G: wild-type cells treated with 25 μM FeSO₄, 25 μM Fe-Gly; treated with 25 μM FeSO₄, 25 μM Fe-Gly. (A) Western blot bands of iron regulators and transporters. Statistical analysis of (B) hypoxia-induced factor-2α (HIF-2α), (C) iron regulatory protein 1 (IRP-1), (D) IRP-2, (E) peptide transporter 1 (PepT1), (F) ferroportin 1 (FPN1), (G) DMT1, (H) iron-regulated transporter (IRT)-like protein 14 (Zip14), (I) ferritin expression.

Expression of some iron regulators and transporters after treated with 25 μM of different iron sources for two hours. Values not sharing a common letter differ significantly (p < 0.05). W: wild-type cells treated with 0 μM iron; W25S, W25G: wild-type cells treated with 25 μM FeSO₄, 25 μM Fe-Gly; K0: DMT1-knockout cells treated with 0 μM iron; K25S, K25G: W0: wild-type cells treated with 0 μM iron; W25S, W25G: wild-type cells treated with 25 μM FeSO₄, 25 μM Fe-Gly; treated with 25 μM FeSO₄, 25 μM Fe-Gly. (A) Western blot bands of iron regulators and transporters. Statistical analysis of (B) hypoxia-induced factor-2α (HIF-2α), (C) iron regulatory protein 1 (IRP-1), (D) IRP-2, (E) peptide transporter 1 (PepT1), (F) ferroportin 1 (FPN1), (G) DMT1, (H) iron-regulated transporter (IRT)-like protein 14 (Zip14), (I) ferritin expression.