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. 2019 Mar 22;20(6):1468. doi: 10.3390/ijms20061468

Figure 4.

Figure 4

Figure 4

CAPE inhibits hypoxia- and PDGF-BB-induced HIF-1α expression through a AKT/ERK pathway in human PASMCs. (A) Cells were cultured for 24 h in normoxia (20%) or hypoxia (3%) with or without CAPE (5 or 10 μM). (B) Cells were pretreated with CAPE for 2 h, and then treated with or without PDGF-BB for 24 h. The HIF-1α mRNA levels were determined by real-time PCR. (C) Cells were cultured for 6 h in normoxia (20%) or hypoxia (3%) with or without CAPE (5 or 10 μM). (D) Cells were pretreated with CAPE for 2 h, and then treated with or without PDGF-BB for 6 h. The HIF-1α expression was determined by Western blot. (E–H) Production of phospho-ERK, AKT, NF-κB and expression of HIF-1α by human PASMCs. Cells were pretreated in the absence of inhibitor or with PD98059 (20 μM), LY294002 (10 μM), or CAPE (5 or 10 μM) for 2h, and then were cultured for 4 h in normoxia (20%), hypoxia (3%), or PDGF-BB. Cell lysates were examined by Western blotting using specific antibodies. Values are the mean ± SEM of three independent experiments. * p < 0.05; ** p < 0.002; *** p < 0.001, as compared with the control group. # p < 0.05; ## p < 0.002; ### p < 0.001, as compared with the cells exposed to hypoxia or PDGF-BB.