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. 2019 Mar 22;20(6):1455. doi: 10.3390/ijms20061455

Figure 1.

Figure 1

MAPK (mitogen-activated protein kinase) phosphoserine/threonine/tyrosine-binding protein (MK-STYX) causes histone deacetylase isoform 6 (HDAC6) to become partly nuclear and decreases HDAC6 aggregates. (A) Representative examples of the subcellular distribution of endogenous HDAC6, detected with an anti-HDAC6 antibody (anti-HDAC6) and anti-rabbit conjugated to FITC, in HEK/293 cells transfected with mCherry (control vector) or mCherry-MK-STYX. We scored the localization of HDAC6 as cytosolic or whole cell (cytosolic and nuclear) in the (B) presence or (C) absence of serum, in HEK/293 cells overexpressing mCherry-MK-STYX or mCherry control plasmid. (D) We scored the number of cells with HDAC6 aggregates that formed in the absence of serum. Paired t-test statistical analysis was performed; the error bars are ±SEM; * p < 0.05 for mCherry compared to mCherry-MK-STYX. Scale bar, 10 μm. The quantified data is the cumulative output of n = 3 independent biological experiments. Taking all fields of view into account, cell density was comparable for cells expressing mCherry and mCherry-MK-STYX. Representative images were chosen to illustrate the subcellular distribution of HDAC6.