FIGURE 1.

Accumulation of ROS induced by waterlogging. (A) DAB staining for H₂O₂ and NBT staining for superoxide anion in the primary root of wild-type Arabidopsis after waterlogging treatment. Bars = 50 μm. (B) DCFH-DA staining for ROS in the primary root of wild-type Arabidopsis after waterlogging treatment. Bars = 50 μm. (C–D) Relative staining intensities calculated from (A–B). (E) H₂O₂ and (F) superoxide anion contents in wild-type roots after waterlogging treatment. All of the experiments have been repeated at least three times. Data shown are the mean ± SD (n = 3). ^∗P < 0.05; ^∗∗P < 0.01; ^∗∗∗P < 0.001 by Student’s t-test.