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. 2019 Apr 11;10:468. doi: 10.3389/fpls.2019.00468

FIGURE 2.

FIGURE 2

Changes in AOX1a expression levels and antioxidant enzymes activities under waterlogged conditions. (A) Western blot analysis of the level of total AOX proteins in wild-type roots under waterlogged conditions. Plant actin below the blots to indicate the amount of protein loaded per lane. (B) Quantitative analyses of the relative gray values of the western blotting. (C) qRT-PCR analyses showing the relative expression of AOX genes (AOX1a, AOX1b, AOX1c, AOX1d, and AOX2) upon waterlogging. (D) SOD, (E) CAT, and (F) APX activities in wild-type roots after waterlogging treatment. All of the experiments were repeated at least three times. Data shown are the mean ± SD (n = 3). ∗P < 0.05; ∗∗P < 0.01; ∗∗∗P < 0.001 by Student’s t-test.