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. 2019 Apr 11;10:468. doi: 10.3389/fpls.2019.00468

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Copyright © 2019 Guan, Lin, Liu, Li, Zhou, Mei, Li and Deng.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Autophagy occurs first in the root stele. (A) Confocal images of primary roots expressing GFP-ATG8e. Roots were counterstained with PI. Bars = 50 μm. (B) Numbers of puncta per root section in the root tips and mature root cells of wild type in (A). (C) The expression of the ATG8epro:GUS reporter was monitored in wild-type roots. Bars = 50 μm. All of the experiments have been repeated at least three times. Data shown are the mean ± SD (n = 3). ^∗P < 0.05; ^∗∗P < 0.01; ^∗∗∗P < 0.001 by Student’s t-test.